SCIENTIFIC ARTICLES

Over one hundred scientific articles covering KROMATON's Fast Centrifugal Partition Chromatography equipments have been published. Below are two non-exhaustive lists of such articles.

  • Scientific articles ranked by purified compounds family
    Articles with FCPC Kromaton by compound family
  • Scientific articles ranked by application fields
    Articles with FCPC Kromaton by application field

 

BOOKS

  • Countercurrent Chromatography: The Support-free Liquid Stationary Phase, Alain BERTHOD, 2002, 422p

    countercurrent chromatography book
  • Preparative Chromatography Techniques: Applications in Natural Product Isolation, Kurt HOSTETTMANN, Andrew MARSTON, Maryse HOSTETTMANN, 1998, 260p

    reparative_chromatography_techniques_applications_in_natural_product_isolation
  • Centrifugal Partition Chromatography, Alain P. FOUCAULT, 1995, 431p

    -	Centrifugal Partition Chromatography, Alain P. FOUCAULT

 

Posters

  • Centrifugal partition extraction as an efficient tool for natural extract fractionation and metabolite screening, Mahmoud Hamzaoui, Sylvain Purson, Romain Reynaud, Jean-Marc Nuzillard, Jean-Hugues Renault, Jane Hubert AFERP (Brussels)                                                                                                                                                        

          AFERP

  • Fractionation of cocoa procyanidins according to the degree of polymerization by centrifugal partition chromatography, Jan A.Glinski, Peter Kinkade, Vitold B. Glinski, Matthew H. Davey, Harvey Stanley, W. Jeff Hurst
    ICNPR 2012 (New York)
    poster5
  • Efficient small volume hydrostatic countercurrent chromatography column, Alain BERTHOD, Nazim MEKAOUI, Jérémy MEUCCI, Karine Faure
    HPLC 2012 (Anaheim), CCC 2012 (Hangzhou)
    poster1
  • Evaluation of Limonene as a possible "green" non polar solvent for alkane replacement, Karine FAURE, Nazim MEKAOUI, Alain BERTHOD
    CCC 2012(Hangzhou)
    poster2
  • Chromatographie à contre courant : nouvelles colonnes hydrostatiques performantes : les colonnes FCPC®, Alain BERTOD, Nazim MEKAOUI, Jean Luc MILESI, Jérémy MEUCCI, Francois de la POYPE, Roger MICHUD, Michel L’KHERBA
    SEP 2011 (Toulouse)
    poster3
  • Large-scale purification of glucosinolates by strong ion-exchange centrifugal partition chromatography from lab-scale CPC to 5L-pilot-scale FCPC, TORIBIO, Leslie BOUDESOCQUE, Benoit PINEL, Michel LAFOSSE, Francois de la POYPE, Jean Marc NUZILLARD, jean Hugues RENAULT
    CCC 2010 (Lyon)
    poster4

Application notes

Application notes of Kromaton's laboratory :

Note titleReferences
Evaluation of retention and pressure with different solvent systems on a Bench scale FCPC200 Application note 04001
Evaluation of efficiency and resolution according to flow-rate and rotation speed Application note 04002
Evaluation of efficiency and resolution according to flow-rate and rotation spee Application note 04003
New 50 ml rotor efficiency and fastness comparison with other FCPC roto Application note 04004
General fractionation of Hypericum perforatum extract on a Bench scale FCPC1000  Application note
High purification of peptide from crude synthetic mixture on Bench scale FCPC200 Application note 04006
Binary fractionation of neoruscogenin and ruscogenin on Bench scale FCPC1000 Application note 04008
Piperine purification : injection capacity test on Analytical scale FCPC50 Application note 04009
Piperine purification : resolutions according to flow rate and rotation speed Application note 04010
Scale up studies with alkyl benzene on 50, 200 and 5000 ml FCPC columns Application note 05012
Scale up on peptide purification from Bench scale FCPC200 to pilot scale FCPC5000 Note d'application 06013
High purification of capsaicine from crude hot pepper extract on analytical FCPC50 Note d'application 06014
Spilanthol purification from Jambu extract on analytical FCPC50 Application note 06015

 

If you wish to access a note that is not available for download, feel free to contact us and we will send it to you shortly.

 

TERMINOLOGY

Centrifugal Partition Chromatography (CPC) is one of the various techniques constituent of Countercurrent Chromatography (CCC).

Centrifugal Partition Chromatography is also known as Hydrostatic Countercurrent Chromatography. This refers to the rotary movement of the column which rotates around one single rotation axis, contrary to Hydrodynamic Countercurrent Chromatography systems that have a planetary rotation movement around two rotating axes.

 

 

WORKING PRINCIPLE

Centrifugal Partition Chromatography is a liquid-liquid chromatography technique. The Kromaton Fast Centrifugal Partitioning Chromatograph thus functions based on the principles of liquid/liquid partitioning chromatography: two immiscible liquid phases are mixed together to form a two-phase system, and are then separated multiple times. The individual solutes are isolated based on the different partitioning coefficients of each compound in this two-phase system.

 

One of the liquid phases of the two-phase system is used as a stationary liquid phase: it is fed into the column (the rotor) while the latter is spinning at moderate rotational speed. The stationary phase is retained inside the rotor by the centrifugal force generated.

 

The second phase of the two-phase system is used as the mobile phase containing the solutes to be extracted. It is fed under pressure into the rotor and pumped through the stationary phase.

 

Both phases are mixed together. It is at that time that the exchange of molecules between the two phases occurs. The separation of the solutes is achieved as a function of the specific partitioning coefficient (Kd) of each solute between the mobile and stationary phases. The mobile phase then decants at each cell outlet thus entering the next cell.

 

The eluted fractions of the mobile and stationary phases are collected over a period of several minutes to several hours. These fractions, or eluates, will contain the individual purified solutes.

 


anim ampoulesquotient Kd

 

The Fast Centrifugal Partition Chromatograph FCPC’s rotor consists of several individual Twin Cell discs, the number of which varies based on the process.

 

shema de pricncipe anglais

 

Seals (gaskets) are positionned between adjacent discs so as to isolate the cells from one disc to the other.

 

The discs are aligned in such a way that all the cells are in series, thus creating several hundred extraction stages.

 

Each cell corresponds to a discrete extraction stage where the two phases are mixed together before decantation.

 

 

APPLICATION FIELDS

Centrifugal Partition Chromatography is a preparative chromatography technique. It is an alternative to classic purification techniques such as preparative HPLC (High Performance Liquid Chromatography) or Flash Chromatography.

Centrifugal Partition Chromatography can be used for:

  • Final purification (purity > 99.5%) of molecules derived from natural extracts, synthetic mixtures or biological matrices
  • Fractionization or pre-fractionization of highly complex crude extracts
  • Liquid-liquid extraction using the newly developed Centrifugal Partition Extractors (CPE) prototypes

 

 

fleche techno

 

APOLAR                                                                                              POLAR

 

Simple lipids - Sterols - Inorganic elements - Polyphenols - Polysaccharides-Proteins
Phospholipids - Various inorganic acids - Anthocyanins - Peptides
Glycolipids - Alkaloids - Flavonoids - α amino acids

 

 

BENEFITS

Because there is no packed solid phase required, Centrifugal Partition Chromatography and the FCPC range offers many benefits:

 

COST EFFECTIVE

  • No solid packing material that is costly to buy, replace or reprocess
  • Low solvent consumption (10 to 25% less)
  • One single rotor adapted to all types of separations

A SOFT TECHNIQUE

  • No irreversible adsorption or sample loss -> 100% recovery
  • No denaturation of fragile molecules due to interaction with silica -> adapted to fragile molecules

EFFICIENT

  • High purification levels up to 99.9%

FASTER

  • 3 to 5 times faster than other current solutions

FLEXIBLE

  • Adapted to all types of products, natural or synthetic
  • Well-adapted to a very wide range of polarities

IDEAL FOR EASY SCALE UP

  • One single separation/purification technique adapted to samples ranging from mg to several kg

 

schema purification